Oxidative stress is a potentially important etiologic factor for many chronic diseases, including cardiovascular disease, neurodegenerative disease, and cancer, yet studies often find inconsistent results. Three of the most widely-used biomarkers of oxidative stress are F2-isoprostanes for lipid peroxidation and 8-hydroxy-2-deoxyguanosine (8-oxo-dG) and the comet assay with FPG for oxidative DNA damage. The aim of this study was to examine associations among three biomarkers of oxidative stress, F2-isoprostanes, 8-oxo-dG, and the comet assay with FPG, in a sample of 135 healthy African American and White adults. The strongest associations were observed between F2-isoprostanes and the Comet tail moment (r=0.22, p=0.01). There were non-statistically significant weak correlations between 8-oxo-dG and the comet assay (r= -0.09), as well as F2-IsoP (r= -0.04). There were also significant differences between the oxidative stress assessment methods by several participant characteristics, including sex, race, physical activity, and passive cigarette smoke exposure. The results of our study are informative, particularly for researchers seeking to compare results pertaining to oxidative stress across studies and/or assessment methods. The development and use of oxidative stress biomarkers is an exciting and promising field, however additional validation studies are necessary to establish accuracy and comparability across studies and oxidative stress assessment markers.

Citation Information: Cancer Prev Res 2008;1(7 Suppl):B85.

Seventh AACR International Conference on Frontiers in Cancer Prevention Research-- Nov 16-19, 2008; Washington, DC