The presence of “M” component, as monoclonal gammopathy of undetermined significance (MGUS) in many patients with psoriatic arthritis and in 5% healthy people older than 70 years is well known. Besides, it was stressed out that patients with psoriatic arthritis and MGUS can sometimes develop multiple myeloma.
Serum from patient 83 years old, with psoriatic arthritis diagnosed at 2000 was analyzed. Determination of the total IgG, IgA and IgM immunoglobulins was done by nephelometry using commercial (Binding Site) tests. Serum protein electrophoresis was done on gel electrophoresis in barbital buffer, pH=8.6; this was followed by immunofixation using sheep antihuman antibodies (Binding Site) to light and heavy chains of immunoglobulins. Determination of serum IgA and IgG immunoreactivity to gliadin in (IU/ml) was done using commercial Binding Site ELISA test, while serum immunoreactivity to cow’s milk protein (CMP) in (AU/ml) was done by home made ELISA test, using cows milk pasteurized powder (ICN Biomedicals, Inc.) as the antigen and sheep antihuman IgA and IgG, HRP labeled antibodies (Binding Site) as secondary antibodies. Blocker was 1% bovine serum albumin.
Results obtained showed the enhanced IgA and IgG immunoreactivity to CMP. The analysis of serum by electrophoresis and immunofixation reveals the presence of monoclonal IgG (λ) immunoglobulin. Upon the knowledge that the enhanced immunoreactivity especially to CMP exists the patient decided to consume the food without cow’s milk proteins. About month and half after the start of CMP free diet he has done his blood for the analysis of serum proteins again. In that time of period the patient has not taken any immunosuppressive drugs.
Electrophoresis of patient sera reveals that after the CMP free diet the “M“component disappeared. These data are supported by the findings that serum anti-CMP IgG dropped from 892 AU/ml to 0 AU/ml (anti-CMP IgA also dropped from 240 AU/ml to 0 AU/ml) Besides, concentrations of total serum proteins were diminished after the CMP free diet from 73 g/l to 69 g/l, while concentration of IgG was decreased from 17.8 g/l to 11.2 g/l. Our report shows that there could be a connection between diet restricted for immunogenic protein(s) and disappearance of MGUS. Our results are along with the data from only one report dealing with a possible link between disappearance of MGUS and gluten free diet. They open the question whether the special, appropriate diet restriction regime could help in the prevention of multiple myeloma.
Citation Information: Cancer Prev Res 2008;1(7 Suppl):B138.
Seventh AACR International Conference on Frontiers in Cancer Prevention Research-- Nov 16-19, 2008; Washington, DC