Abstract A5: Inhibition of tumor cell migration and angiogenesis by sulphated polysaccharides from Sargassum wightii (Greville)

A5

Seaweeds are potential renewable resources of the marine environment. They are low in fats but contain vitamins and bioactive compounds like terpenoids and sulphated polysaccharides. Sulphated polysaccharides from brown algae are found to exhibit diverse biological activities like anticoagulant, antihyperlipidemic, antiviral, antioxidant, antitumor, antimetastatic and antiangiogenic activities. In this study, the antiproliferative, antiinvasive and antiangiogenic properties of sulphated polysaccharides from brown seaweed, Sargassum wightii (Greville) against colon carcinoma cell line COLO 320DM were investigated. Crude sulphated polysaccharide was extracted from S. wightii collected from Mandapam coast, South east coast of India. The antiproliferative activity of the seaweed polysaccahrides on COLO 320DM was evaluated by MTT assay and DNA fragmentation studies. The inhibitory potential on the migration of this invasive cell line was assessed by wound healing assay. The effect of sulphated polysaccharides against angiogenesis was investigated by chorio allantoic membrane (CAM) assay in chick embryo. Sulphated polysaccharides from S. wightii exhibited moderate growth inhibitory activity against COLO 320DM at a concentration of 1mg/ml at 24 hours. DNA fragmentation studies revealed characteristic ladder formation which indicates that seaweed polysaccharides have effected the induction of apoptosis. The seaweed extract was found to inhibit the migration of the colon carcinoma cell line. The antiinvasive property increased with increasing concentrations. However, the activity was more or less similar at 24 and 48 hours. The CAM assay showed that sulphated polysaccharides from the brown seaweed exhibited promising activities against neoangiogenesis. These results clearly indicate that sulphated polysaccharides from brown algae are potential molecular leads for antimetastatic drug development.

Citation Information: Cancer Prev Res 2008;1(7 Suppl):A5.