Objectives: Hypoxia occurs during development of cervical cancer and is considered to correlate with its malignancy. We found that hypoxia induced miR-100 whose expression was low in cervical cancer. We sought to determine the target molecules during hypoxia in cervical cancer.

Methods and Results: We found that the expression of miR-100 was significantly higher under hypoxic condition (1% O2) by realtime RT-PCR. According to the results of in silico analysis, miR-100 targeted mechanistic target of rapamycin (mTOR). Hypoxia and overexpression of miR-100 respectively decreased the activity of luciferase-reporter containing the 3’-untranslated region (UTR) of mTOR with the predicted miR-100-binding site in cervical cancer cell lines, CA and HeLa. By Western blot analysis, hypoxia downregulated the endogenous mTOR protein expression. mTOR mRNA level was also downregulated by hypoxia using realtime RT-PCR.

Conclusions: Our results suggest that miR-100 and mTOR could be a therapeutic target for cervical cancer.

Citation Format: Shigeatsu Takamizawa, Zenta Yamanaka, Hirotaka Nishi. Hypoxia mediates downregulation of mTOR via miR-100 in cervical cancer cells [abstract]. In: Proceedings of the AACR Special Conference on Tumor Immunology and Immunotherapy; 2018 Nov 27-30; Miami Beach, FL. Philadelphia (PA): AACR; Cancer Immunol Res 2020;8(4 Suppl):Abstract nr B38.