Tumors compass remarkably diverse cell populations as a result of genetic and environmental influences. Macrophages are a type of white blood cell with pivotal roles in immunity and tissue homeostasis that were reported to constitute up to 50% of the tumor mass. Macrophages also present a central line of defense against cancer by preventing inflammation as well as specific elimination of tumor cells. However, tumors can manipulate macrophages to switch their function from tumor-suppressing to tumor-supporting. Tumor-supporting macrophages are pro-angiogenic, immunosuppressive and aid the neoplastic progression. My aim is to characterize the core transcriptional network of “supporting” or “repressing” tumor associated macrophages in mice and humans. At phase 1 of the project, I am testing methods to differentially analyze cell populations from tumors with minimal perturbations as well as ways to reduce input for GROseq and RNAseq analysis.
Citation Format: Sascha H. Duttke, Christopher K. Glass. Differential transcriptional profiling of tumor cell populations [abstract]. In: Proceedings of the Second CRI-CIMT-EATI-AACR International Cancer Immunotherapy Conference: Translating Science into Survival; 2016 Sept 25-28; New York, NY. Philadelphia (PA): AACR; Cancer Immunol Res 2016;4(11 Suppl):Abstract nr A147.