Hallmarks of Resistance to Immune-Checkpoint Inhibitors

The clinical development of immune-checkpoint inhibitors (ICI), specifically those targeting the T cell–inhibitory receptors cytotoxic T lymphocyte antigen-4 (CTLA-4) and programmed death-1 (PD-1), radically shifted the cancer treatment paradigm and transformed oncology practice. Survival outcomes from large, randomized trials with at least 4 to 5 years' follow-up are known for melanoma and non–small cell lung cancer (NSCLC) populations. Reported outcomes from melanoma trials approximate the 4-year survival rate of patients treated with the anti–CTLA-4 therapeutic ipilimumab between 19% and 36%, the anti–PD-1 therapeutic nivolumab at 46%, the anti–PD-1 therapeutic pembrolizumab at 44%, and the combination of nivolumab plus ipilimumab at 53% (1–6). Similar trends are emerging in the NSCLC population treated with anti–PD-1 monotherapy, where the estimated 5-year survival rate of previously treated patients is approximately 16% with nivolumab (CM209-003), 15% with pembrolizumab (KN-001), and of treatment-naïve patients is 23% with pembrolizumab (KN-001; refs. 7, 8). Most recently, it was reported that first-line NSCLC patients with a programmed death ligand 1 (PD-L1) tumor proportion score of at least 50% demonstrated a median overall survival (OS) of 26% (KN-024; ref. 9). Although such outcomes are impressive, most patients with advanced cancer treated with anti–CTLA-4 or anti–PD-1/anti–PD-L1 [anti–PD-(L)1] agents do not achieve durable benefit and show resistance to therapy between 3 months to 3 years after initiating treatment.

Clinical progression on ICIs has been broadly conceptualized into three categories aligned to clinical response scenarios, namely, primary resistance (no response to checkpoint inhibition), adaptive resistance (functional antitumor response that is limited or shaped by immunosuppression), and acquired resistance (initial response followed by eventual disease progression; refs. 10–12). Although these categories closely approximated clinical observations, a deeper understanding of immune resistance necessitates a more expansive framework that can accommodate both dynamic biological complexity and clinical response outcomes.

The approach necessary to derive a resistance framework that will guide future research and facilitate reverse translation is likely to include a systematic characterization of resistance to ICIs, where molecular and clinical definitions are integrated into a structure that can be utilized to identify therapeutic interventions. Critical elements of such a resistance framework should include (i) identification of resistance patterns, (ii) characterization of patterns to define discrete resistance phenotypes, (iii) relational mapping between individual resistance mechanisms and resistance phenotypes, and (iv) integrated validation of resistance phenotypes to clinical outcomes. As part of a workshop sponsored by the Parker Institute for Cancer Immunotherapy (PICI), the Cancer Research Institute (CRI), and the Society of Immunotherapy of Cancer (SITC), we constructed a view of immune resistance by aggregating the evidence of known resistance mechanisms that undermine key biological processes and block successful antitumor immunity. We organized the evidence into eight biological converging points and denote them as immune-resistance nodes, collectively representing the emerging hallmarks of resistance to ICIs (Fig. 1). For each immune-resistance node, we examined the seminal discoveries that define each node and posed critical questions (Table 1), which if answered would greatly expand our notion of resistance. We then speculated on how these core immune-resistance nodes might integrate with additional aspects of malignancy, including tumor development, treatment modalities, detection of resistance, and approaches to target resistance (Fig. 2). Although in this Perspective article we interchangeably utilize the terms checkpoint inhibitors, ICIs, immunotherapy, and immune resistance, our focus is centered around resistance to anti–PD-(L)1 and anti–CTLA-4 therapies and predicated primarily on clinical and translational data from blockade of PD-1 and PD-L1. We recognize that anti–PD-(L)1 and anti–CTLA-4 ICIs work through distinct mechanisms but we lack balanced clinical and translational data sets to delineate whether they may be provoking distinct resistance mechanisms and resistance patterns. As monotherapy, anti–PD-(L)1 agents have demonstrated clinical efficacy across a wide range of tumor indications, which makes it possible to examine the range of resistance. Notwithstanding, this question remains vitally important for future work, especially because both anti–PD-(L)-1 and anti–CTLA-4 ICIs are now used in combination regimens with each other and with other drugs that may elicit unique resistance patterns.

Oncogenic signaling is the least understood aspect of functional immunogenicity, but has a dramatic influence on the tumor microenvironment (TME) and normal tissue surrounding the tumor mass. Evidence for key oncogenic processes that can potentially foster resistance to immunotherapy includes: (i) oncogenic cooperation, (ii) exclusion of CD8⁺ T cells, (iii) induction of regulatory T cells (Treg) and myeloid-derived suppressor cells (MDSC), and (iv) deregulation of IFNγ signaling and PD-L1 expression.

Oncogenic cooperation and the coevolution of tumors and their immune environments were elucidated in recent analysis from The Cancer Genome Atlas (TCGA) database that landscaped 10 canonical cancer pathways (13). This exploration of mutually exclusive and co-occurring pathways indicated the potential influence of oncogenic synergies that can promote treatment resistance and potentially immune evasion. Oncogenic cooperation between Kras and Myc to produce highly proliferative tumors with an inflamed, angiogenic, but immunosuppressed stroma was also demonstrated using a mouse lung cancer model of conditional Kras^G12D and inducible Myc (14), indicating the ability of Myc overexpression to rapidly clear the tumor of innate and adaptive immune lymphocytes. Several reports suggesting that Myc gain of function can influence antitumor immunity and immunotherapy response further substantiate these findings (15–18).

Oncogene-driven T-cell exclusion is another process by which tumors can shut down antitumor immunity and is evidenced by gain-of-function alterations of the β-catenin–Wnt pathway. Analyses of human melanoma samples revealed that upregulation of β-catenin–Wnt signaling was associated with a lack of T cells in both the primary tumors and distant metastases (19, 20). Analyses in a conditional Braf^V600E and inducible β-catenin mouse model revealed a causal relationship between activation of β-catenin signaling, lower T-cell infiltration, and lack of response to checkpoint inhibition (21–24). Additionally, oncogenic signaling can induce suppressive immune-cell populations as exemplified by the potential of oncogenic KRAS to convert CD4⁺ T cells to functional Tregs (25) and the inactivation of p53 to induce MDSCs (26). Similarly, the hedgehog pathway can have profound impact on the composition and functionality of tumor-infiltrating immune cells and upregulation of PD-L1 expression (27–29). The activity of oncogenes, particularly members of the receptor tyrosine kinase (RTK)–RAS pathway, has recently been linked more directly to immunosuppression. RAS signaling can upregulate PD-L1 expression on tumor cells by stabilizing PDL1 mRNA (30), an observation that is consistent with the reported association of KRAS expression with increased PD-L1 expression suggesting that the KRAS–IRF3 pathway can support immune suppression and resistance to immunotherapy (31).

Clinical trial observations indicate that patients whose tumors harbor driver mutations do not respond well to ICIs (e.g., KRAS mutations in colorectal carcinoma and EGFR mutations in NSCLC), although melanoma tumors with either BRAF^V600E or BRAF^WT alleles potentially respond similarly to anti–CTLA-4 or anti–PD-(L)1 agents. Careful analysis of emerging data sets for a better understanding of the local and systemic impact of driver mutations in the context of ICI therapy is key.

Human leukocyte antigen (HLA)–directed antigen presentation is the basis for T cell–mediated antitumor responses. Substantial evidence suggests that tumor cells can evolve to escape immune recognition by reducing HLA proteins on their surface (HLA loss), thus creating an important avenue for ICI resistance (32, 33).

Genetic (“hard”) and epigenetic (“soft”) mechanisms leading to HLA loss can directly affect HLA class I including its transcriptional regulation, or indirectly target genes that are part of the antigen-presentation machinery (APM). In a recent study from the TRACERx consortium analyzing 258 tumor regions from 88 prospectively acquired tumors, 56% of lung adenocarcinomas and 78% of lung squamous cell carcinomas showed evidence of antigen-presentation disruption, through either HLA loss of heterozygosity (LOH) or APM mutations (34). Complete loss of beta-2 microglobulin (B2M) alleles, an essential component of major histocompatibility complex class I (MHC-I) required for antigen presentation, was evident only in ICI nonresponders, whereas permutations of the B2M gene (absence of tumor-specific expression, frameshift mutations, or LOH) were observed in ∼30% of ICI nonresponders (35).

Epigenetically, HLA gene expression in tumor cells can be regulated directly through promoter hypermethylation. HLA class I gene promoter methylation occurs in 49% to 60% of gastric cancer samples, compared with 6% to 19% of adjacent normal tissue, and often results in transcriptional inactivation of MHC-I loci HLA-A, -B, and -C (36). Indirect epigenetic regulation of HLA gene expression, through histone methyltransferases within chromatin remodeling machinery, can subsequently impact a range of potential resistance mechanisms including T-cell infiltration, TNFα responsiveness, PD-L1 expression, and MHC-1 expression (37). Similarly, expression of the HLA-A, -B, and -C genes, and B2M are under at least partial control of the transcription factor DUX4, which is normally suppressed epigenetically after development. In patient subsets across tumor types, genetic variation can trigger dysregulated DUX4 expression, leading to reduced IFNγ responsiveness and decreased MHC-I expression (38).

Although evidence of HLA loss has been documented (32), a systematic evaluation of the combined frequency and contributions of hard and soft HLA loss across tumor types is warranted to expand our understanding of disease etiology. Hard HLA loss, HLA heterogeneity, as well as previously reported HLA types, were not found to be associated with response in a recent meta-analysis (39). It is likely that we need to capture all mechanisms of HLA downregulation to accurately estimate their impact on outcomes across treatment settings and devise strategies to reverse immune resistance, or possibly prevent a tumor from evolving an HLA loss phenotype. Additionally, partial or complete loss of HLA proteins on tumors spotlights natural killer (NK) cells and their role in immunosurveillance. As NK cells detect HLA class I expression loss in infected or transformed cells and initiate target cell killing (40), tumors displaying HLA loss must also have evolved resistance against NK cell–mediated killing (41). Thus, exploration of NK cell–based therapies might extend immunotherapeutic options that do not require neoantigen presentation by HLA.

Tumor antigens can arise from multiple sources (42). The productive engagement of the T-cell receptor (TCR) complex requires not only the presentation of antigens but also immunologically suitable antigens in sufficient quantities to produce effective T-cell targeting and tumor killing. Thus, antigen suitability and antigenic escape can affect resistance to immunotherapies. Recent focus on the evaluation of neoantigens as therapeutic targets has garnered attention from the following observations: (i) lack of thymic deletion of T cells targeting neoantigens relative to deletion of T cells targeting self-antigens (43), (ii) association of predicted neoantigen number with OS and/or clinical response (44–49), (iii) successful therapeutic neoantigen vaccination in mice and evaluation in patients (50–52), and (iv) evidence of clinical responses using neoantigen-specific T cells in adoptive transfer (53, 54).

Lack of ICI response has been associated with heterogeneous neoantigen expression and a lack of truncal neoantigens (34, 46, 55), even though only a minority of predicted neoantigens are truly immunogenic in vivo (56–59). Tumors displaying either few or ample neoantigens can succumb to immune-editing mechanisms selecting for tumor clones with diminished neoantigen expression (60). In fact, it is possible that primary tumors may already display a resistance phenotype determinately characterized by a paucity of immunogenic neoantigens. As discussed above, immunotherapy resistance can be mediated via HLA loss and possibly enriched in tumors with immunogenic neoantigen expression (61, 62). A fascinating observation from preclinical neoantigen vaccination experiments demonstrates that neoantigens predicted based on MHC-I binding characteristics can stimulate CD4⁺ T-cell responses in excess of CD8⁺ T-cell responses (50).

A complete view of cancer antigens through tumor antigen expression mapping will enable an assessment of the character and suitability of antigens to trigger productive ICI-directed antitumor immunity. Adoptive transfer studies using tumor-infiltrating lymphocytes (TIL) have shown that T cells enriched for a single dominant neoantigen can elicit responses in patients with metastatic cancer. A neoantigen map together with a computational estimate of the number of neoantigen specificities required to promote immune responses will provide a baseline for understanding antigen suitability. Thus, our ability to better define neoantigens and their role in maximizing antitumor immunity should enable approaches to prevent or reverse ICI resistance and hopefully improve clinical outcomes.

T cell–mediated antitumor immune responses are key to successful immunotherapy, and their dysfunction is central to ICI resistance. In the context of chronic viral infections and malignancies (63, 64), exhausted T cells gradually lose their functional capacities, most notably through chronic antigen exposure, upregulation of immune-checkpoint receptors, and incomplete priming. This T cell–exhaustion program is emerging as a specific differentiation pathway enabled by the HMG-box transcription factor TOX via specific epigenomic remodeling; it normally serves to ensure survival of antigen-experienced T cells during chronic stimulation by preventing TCR overstimulation and activation-induced cell death (65–67). Interestingly, genetic inactivation of TOX in chronically activated T cells does not necessarily promote sustained T-cell functionality, likely due to enhanced cell death and premature loss of TOX-deficient T cells (65, 67). Similarly, in tumors, although it is possible to transiently counteract the exhaustion of chronically stimulated tumor antigen–specific CD8⁺ T cells, the “rescued” CD8⁺ T cells often reenter a dysfunctional state (68, 69). PD-1 blockade specifically acts by reactivating partially exhausted progenitor-like TCF-1⁺PD-1⁺CD8⁺ T cells, which can mature and execute their function, but ultimately become terminally exhausted themselves (69–71). Paradoxically, these observations indicate that blocking the PD-1 pathway can accelerate the exhaustion process in progenitor-like tumor-specific T cells, leading to ICI resistance.

A key question is whether dysfunctional T cells can be epigenetically reprogrammed or alternatively eliminated to make space for new, functionally stable, tumor antigen–specific T-cell populations. Additionally, PD-1 blockade of suboptimally primed CD8⁺ T cells can induce a specific population of dysfunctional T cells (PD-1⁺CD38^hiCD8⁺), suggesting that ICI resistance can be reversed with functional antigen stimulation and implying a temporal relationship between optimal priming and subsequent PD-1 blockade (72).

In addition to TCR engagement of a cognate peptide–MHC complex (pMHC), costimulation mediated by canonical CD28–CD80/86 engagement is critical for a productive T-cell response and for long-lasting antitumor immunological memory. Lack of costimulation during TCR–pMHC recognition has been implicated in T-cell anergy and hyporesponsiveness. Evidence for functional T-cell hyporesponsiveness suggests that it occurs under the influence of either chronic antigen stimulation or TCR engagement lacking costimulation (73). It is plausible that inadequate expression of costimulatory molecules in antigen-presenting cells in the presence of a progressing tumor may contribute to ICI resistance. Theoretically, this may be further exacerbated by chronic TCR stimulation of TME-residing T cells, ultimately leading to T-cell anergy (74–76). For example, in a tamoxifen-induced liver cancer model, naïve antigen-reactive T cells displayed hyporesponsiveness 1 to 2 days after transfer, due to upregulation of negative transcriptional regulators, including those associated with T-cell anergy and hyporesponsiveness (Batf, Egr1, Prdm1, Ptpn11, Ptpn12, Dusp1, and Dusp6) and inhibitory cell-surface receptors (Pdcd1, Lag3, Cd160, and Ctla4; ref. 77). The inability of NFAT–AP-1 transcriptional complexes to drive T-cell activation is a common feature of anergy induced by lack of costimulation and checkpoint-induced immune dysfunction (78). NFAT signaling can also drive pathways associated with T-cell exhaustion and anergy in the absence of AP-1 (79, 80), highlighting distinct immune resistance pathways governed by this transcription factor.

Additional costimulatory pathways including 4-1BB have been known to compensate for CD28 signaling (81), and both CD28 and 4-1BB have been used as costimulatory domains in the development of chimeric-antigen receptors (CARs; ref. 82). Ultimately, delineating the role of costimulation from immune agonism will expand our understanding of T-cell activation and possibly identify new strategies to prevent the influence of hyporesponsiveness and anergy on ICI resistance. Ongoing clinical studies, using monoclonal or bispecific antibodies triggering costimulatory targets, will help elucidate the importance of costimulation in the context of treatment resistance.

IFNγ, the quintessential effector cytokine of Type I immunity, is produced by activated cytotoxic CD8⁺ T cells, γδ T cells, and NK cells and can exert pleiotropic effects. IFNγ signaling through IFNGR1–IFNGR2 complexed heterodimers recruits JAK1 and JAK2 and leads to STAT1 phosphorylation. Phosphorylated STAT1 modulates IFNγ-regulated gene expression (including genes encoding MHC-I, APM, and costimulatory molecules), induces the expression of CCL9 to recruit CD11b⁺ dendritic cells, skews macrophages toward an M1 proinflammatory phenotype, and inhibits immune suppressive cells including Tregs, Th2, Th17, and MDSCs (83–86).

Mice lacking IFNγ, IFNγ receptors, or STAT1 develop tumors more rapidly and with increased frequency, demonstrating the central role of IFNγ signaling in tumor surveillance and antitumor immunity (87). IFNγ acts directly on tumor cells to enhance tumor cell immunogenicity through MHC-I upregulation and plays a role in tumor escape by inducing tumor cells to become selectively unresponsive to IFNγ. In melanoma, acquired resistance to PD-1 blockade is associated with IFNγ-pathway defects involving truncating mutations in JAK1 and JAK2 (88). In adaptive resistance, IFNγ can potentially compromise antitumor immunity by directly upregulating both PD-L1 and PD-L2 on tumor and immune cells (48, 89, 90) and can drive resistance to checkpoint blockade by PD-L1–dependent and –independent mechanisms (91). Furthermore, interrupting chronic IFNγ signaling in tumor cells leads to increased IFNγ production by exhausted T cells and enhanced IFN-stimulated gene expression in tumor and immune cells. As expected, tumors with adequate MHC-I and antigen are killed by T cell–mediated mechanisms that produce IFNγ, and tumors with low expression of MHC-I or limited antigen are killed by PD-1⁺TRAIL⁺ NK cells receiving maturation signals from IFNγ (92). Despite divergent roles for IFNγ signaling, altering these pathways can enhance response to ICIs (92), and IFNG RNA gene-expression signatures have been shown to predict response and survival with anti–PD-1 therapy (93).

T-cell memory, in particular tissue-resident memory (TRM) CD8⁺ T cells, may be a crucial mediator of antitumor immunity and ICI resistance. Understanding the mechanisms and conditions needed to improve the generation and maintenance of CD8⁺ T-cell immune memory against tumors is critical for the improvement of immunotherapy outcomes, especially if immune memory can yield durable complete responses.

The importance of TRM CD8⁺ T cells is supported by data in patients with melanoma who responded to anti–PD-1 and harbored expanded CD8⁺ effector memory T-cell populations (94) and in patients with breast cancer whose TRM CD8⁺ T cells contributed to immunosurveillance (95). Epigenetic and genomic studies characterizing the CD8⁺ TILs indicated that a subset of these cells have a progenitor-exhausted phenotype, resembling stem cell–like memory cells, characterized by TCF-1 expression and associated with clinical benefit to anti–PD-1 therapy (96). Furthermore, although CD8⁺ T cells are viewed as key effectors in immune-mediated tumor rejection, as their presence and persistence have been correlated with therapeutic outcome, it is important to recognize that a productive and long-lasting antitumor immune response likely involves the cooperation of multiple immune cells working in concert with CD8⁺ T cells (97, 98). Thus, fully understanding T-cell memory generation and T-cell persistence will likely help us better select patients suitable for ICI therapy by estimating the quality, depth, and duration of their tumor response and alternatively predict ICI therapy resistance.

As we consider the influence of local tumor processes with systemic effects, inflammation is a key culprit influencing pro- and anti-immune signals that can shape a patient's response or resistance to immunotherapy. In the context of the TME, three clinically relevant tumor–immune phenotypes have been described: (i) inflamed tumors, which are regarded as “hot,” and two categories typically referred to as “cold,” (ii) immune-excluded, and (iii) immune-desert tumors. Undoubtedly, a continuum of immunogenic potential exists, broadly bookended by “inflamed” and “noninflamed” phenotypes (99, 100). The noninflamed “cold” phenotypes probably characterize most human tumors; thus, their clinical significance warrants additional probing to specifically elucidate the origin and nature of the inflammation, or lack thereof, needed to direct and sustain antitumor ICI responses. A nuanced systematic approach that expands the classification of cold tumors according to their inflammatory status may identify cancer-promoting inflammation more clearly, an idea supported by evidence from large trials where nonsteroidal anti-inflammatory drugs such as aspirin reduce cancer incidence and/or mortality (101–103). Elucidating complex relationships between tumor heterogeneity, TME plasticity, and the inflammatory process may reveal how systemic or local inflammation can shape the plasticity of tumor, stromal, and immune cells (103), and it may highlight priority areas likely to influence future clinical research (104).

A specific and critical element of the definition of cold tumors is the absence of functional effector T cells in the TME. Dysfunctional T-cell trafficking and infiltration, therefore, impinge on the necessary inflammation that, together with PD-L1 expression, marks the hot or inflamed tumor–immune phenotype and immunotherapy responsiveness (105–110). The process of functional T-cell trafficking and infiltration faces three major hurdles: (i) defective cellular homing, (ii) regulatory immune cells, and (iii) damaging chemokine networks. The successful homing of T cells is controlled spatiotemporally through tethering and rolling, chemokine activation, and transmigration, all of which are disrupted in tumors (110–112). Some examples leading to dysfunctional T-cell migration include upregulation of expression of the adhesion molecule α4β7, induction of various costimulatory molecules, loss of CCR7, and, possibly, downregulation of retinoic acid (113–117). Upon successful intratumoral homing, lymphocytes encounter physical barriers to infiltration via encounters with Tregs, MDSCs, immature dendritic cells, macrophages, and neutrophils, fibroblasts, and abnormal extracellular matrix. Recent data suggest that it may be possible to shift cellular populations in the TME or reprogram cell types that influence ICI resistance. Macrophages, for example, can switch from an antitumorigenic to a protumorigenic phenotype, thus continuing work has examined the reeducation of M2-like macrophages to M1-like antitumor macrophages using LXR agonism, IL8 inhibitors, and CD40 agonism (118, 119). Although ambitious, the reeducation or plasticity of Tregs and other cell types promoting resistance is promising (120).

Immunometabolism represents a core process with local and systemic influence that may directly or indirectly modulate response and resistance to ICI treatments. Metabolic signaling dictates the function of immune cells (121–125) and thus presents an avenue for identifying clinically relevant immune resistance patterns (126–129). In the hostile hypoxic TME, oxygen and nutrient constraints lead cancer cells to switch from oxidative phosphorylation to aerobic glycolysis, and they then outcompete T cells for glucose (130–132). Checkpoint blockade mediates reduced glycolysis and increased effector T-cell function (129), indicating that the key metabolic mediators ATP and NAD⁺ and the ectoenzymes governing their catabolism may play a role to influence immune resistance (133–136).

T-cell metabolic programming determines T-cell lineage commitment and differentiation (137), with important implications for strategies using ex vivo cultures for adoptive transfer. Furthermore, immunometabolic attributes of CAR T cells influence their persistence and lack of exhaustion. For example, CAR T cells with 4-1BB domains promote central memory T-cell growth, optimizing their metabolic fitness in the TME (138), whereas glucose-limiting expansion is counterproductive, leading to more cytotoxic T cells with increased in vivo persistence and longevity (139). 4-1BB costimulation also increases transfer efficiency and PD-1 blockade effectiveness by increasing mitochondrial mass and the respiratory capacity of cytotoxic T cells (140). Conversely, CAR T cells with CD28 domains yield effector memory T cells with enhanced glycolysis (138). Interestingly, antigen-directed asymmetric cell division generating effector and memory T cells (141) may be overcome by infusing patients with differentially weighted immunometabolic T cells, ensuring immediate antitumor activity and long-lived persistence. These and other insights prompt the clinical investigation of immunometabolic targets with the potential to counteract immune resistance. Innovative combinations of immunotherapies with immunometabolic reprogramming agents hold promise for promoting immune activation, response durability, and, perhaps, mitigation of ICI resistance. Distinguishing metabolic signatures of tumor cells from infiltrated T cells may also provide insights to pinpoint metabolic heterogeneity and metabolic signatures that can alter and override ICI resistance.

The human microbiome is a complex aggregate of microorganisms that exert influence crucial to the metabolic, immunologic, hormonal, and homeostatic functions of the host. The microbiome's effects on ICI resistance remain underexplored. Recent work in mouse models and human studies highlights the impact of gut and tumor microbiota on the potential for clinical response to anticancer therapeutics (142–148). Early work revealed that antibiotics taken within the month preceding the start of ICIs reduced both progression-free survival (PFS) and OS across many metastatic and stage III malignancies (142, 149). Additional studies revealed that fecal microbiota transplantation of stools from patients who were likely to respond or be unresponsive to ICI-based therapy conferred sensitivity or resistance, respectively, to tumor-bearing mice treated with anti–PD-1 antibodies (150). Efforts to better understand gut microbiome signatures associated with ICI resistance include shotgun metagenomic sequencing of patient stools at diagnosis, with samples currently being evaluated in metastatic melanoma (148), kidney (149), and lung cancers (142).

Conversely, ICIs have been shown to affect the composition of the gut microbiome, with one study reporting that anti–CTLA-4 antibodies resulted in an increase in the relative abundance in Bacteroides spp. (B. fragilis) and Burkholderiaceae family members in the ileum. These bacterial families are involved in the IL12-dependent priming of Th1 cells, hence the immunostimulatory and anticancer effects of CTLA-4 blockade (145). It is possible, therefore, that disruptions in gut homeostasis lead to microbial translocation and augmented function in CD8⁺ T cells. These data suggest that microbial translocation from the gut results in the release of danger signals (e.g., Toll-like receptor ligands) triggering tumor regression (151–153). This is consistent with research demonstrating that bacterially derived products can activate the innate immune system and trigger tumor regression (154, 155). These studies and others suggest that the intestinal microbiota modulate the effects of ICIs via bacterial translocation of selected species into secondary lymphoid organs (147). Continued research on the microbiome and the application of clinically actionable hypotheses focused on patients showing resistance to ICIs holds great promise.

Systemic host immunity, not just effector T-cell functionality, inevitably shapes the emergence of a primary tumor and ultimately the potential for response to immunotherapy. In the seminal framework for the role of host immunity in malignant disease, the term “immunoediting” was coined to describe the biological process of immunosurveillance executed through the elimination, equilibrium, and escape phases (156). These principles allow us to explore whether host immunity functions to calibrate responses to checkpoint inhibition and whether there is a relationship between immune capacity and ICI resistance. To better understand all that is at play, a longitudinal systems biology approach in healthy and cancer populations is needed, as well as the means to integrate data sets into meaningful models for testing.

Aged hosts are indicators for declining immune function as measured by redistribution of immune-cell populations, decreases in T-cell functionality, and increases in proinflammatory and immune suppressive cells (157). Additionally, the effects of host germline allelic variation and resistance to therapy suggest that individual immune capacity may be an underexplored variable of immune resistance as patient-specific HLA-I genotypes influence response to anti–PD-1 and anti–CTLA-4 therapy (as described above). Genetic variability conferred by single-nucleotide polymorphisms (SNP) also influences outcomes, with evidence from multiple studies showing that specific SNPs within clinically relevant genes can influence response or resistance to immunotherapy, and even on-treatment hyperprogression. In a study of patients with NSCLC treated with nivolumab, 2 of 7 PDL1 SNP markers were associated with objective response rate and PFS. Both outcomes were improved in patients with G/G and G/T genotypes compared with the T/T genotype at the marker rs2282055/PDL1 (158). By looking for SNPs in multiple genes, including PDL1, PD1, IDO1, and VEGFR2, additional likely scenarios were found where specific SNPs (rs2282055/PDL1 and rs1870377/VEGFR2) might influence accelerated tumor growth in response to ICIs, with perhaps an increased risk of hyperprogression in G/G and G/T genotypes compared with the T/T genotype at rs2282055/PDL1 (159). Although these data carry the limitations of retrospective analyses, they highlight the unfavorable versus favorable alleles in host baseline immunogenetics. Similar approaches investigated SNP analysis in genes associated with autoimmunity but did not find associations between polymorphisms and toxicity. Future work that prospectively and categorically analyzes host immunogenomics will expand our understanding of the systemic immune response and ICI resistance.

In this Perspective, we highlight the importance of systematically investigating immune resistance and integrating large data sets into a framework that will allow us to identify resistance patterns that typically interfere with a patient's ability to respond to ICI therapy. Substantial ongoing efforts to address resistance to checkpoint inhibition have highlighted that immune resistance is driven by diverse, complex, interconnected, and evolving mechanisms. The importance of any one individual immune resistance mechanism, however, is isolated and difficult to interpret without biological context linked to clinically meaningful immune resistance. Initial progress has been made following a recent recommendation by the SITC Immunotherapy Resistance Taskforce (160) calling for the implementation of meaningful definitions of PD-(L)1 resistance with harmonized utility across clinical trials. The SITC Immunotherapy Resistance Taskforce is currently engaged in ongoing work to propose working definitions for immunotherapy resistance in the context of combination regimens including anti–PD-(L)1 plus anti–CTLA-4, anti–PD(L1)1 plus chemotherapy, and anti–PD-(L)1 plus VEGF inhibitors. Harmonization of clinical definitions across trials will allow us to better catalog immune resistance and systematically delineate resistance mechanisms and patterns not only for different ICI classes but also for different ICI-based combination therapies. Biological definitions of resistance are equally critical, and a systematic study of how the biology of the immune resistance process is organized will provide meaningful data that can help identify resistance pattens, resistance phenotypes, and, most importantly, a framework that will allow us to map this information to clinical approaches that can be tested in patients.

To break down this complexity, we carried out a thought exercise, assuming that individual resistance mechanisms converge into core biological processes and postulating that immune resistance might follow biological patterns that may be expressed as discrete phenotypes. We identified seminal data that have thus far informed our understanding of immune resistance and organized the data into eight biological processes we named immune resistance nodes. Collectively, we propose that the eight immune-resistance nodes represent the emerging hallmarks of resistance to ICIs (Fig. 1). We then outlined specific questions that, if answered, would greatly expand our view of immune resistance and could possibly generate hypotheses to examine in reverse translational efforts and incorporate prospectively into clinical trials (Table 1). These questions require the application of high-dimensional multiomic measurements matched to patient-derived samples taken at time points representative of both the disease course and signs of treatment resistance (Fig. 2).

Given the resources required to generate, analyze, and integrate such data sets, ensuring broad accessibility to data is critical. Technically, we need data storage, data sharing, and analysis infrastructures that can integrate these data to drive collective insights. A field-wide oncology effort, with robust harmonization and data sharing among all sectors, is needed to establish a data-driven “real-time” resistance framework that can be systematically tested, analyzed, and iterated within the context of clinical scenarios. Although initially difficult, a contained pilot study would greatly inform future steps and chart the path toward achieving improved survival in patients with cancer.

A.P. Cogdill reports other support from Immunai and personal fees and other support from Vastbiome outside the submitted work. P.B. Robbins reports other support from Pfizer during the conduct of the study; other support from Pfizer outside the submitted work. L.H. Butterfield reports the following unrelated advisory activities: StemImmune/Calidi Scientific and Medical Advisory Board, April 6, 2017–present; Western Oncolytics, Scientific Advisory Board, 2018–2021; Torque Therapeutics, Scientific Advisory Board, 2018–2020; Khloris, Scientific Advisory Board, 2019–present; Pyxis, Scientific Advisory Board, 2019–present; Cytomix, Scientific Advisory Board, 2019–present; DCprime, Scientific Advisory Board meeting, Nov. 2020; RAPT, Scientific Advisory Board, 2020–present; Takeda, Scientific Advisor, 2020–present; EnaraBio scientific advisor, Feb. 2021. A. Cessano reports being a full-time employee and stock owner of ESSA Pharma. Consultant for NanoString, Arch Oncology, and Bayer. None of the above relationships constitute conflict of interest. C. Hammer reports other support from Genentech/Roche outside the submitted work. C.L. Haymaker reports other support from Briacell Therapeutics and personal fees from Nanobiotix outside the submitted work; in addition, C.L. Haymaker has a patent for TLR9 modulators for treating cancer. pending. C.E. Horak reports personal fees from Bristol Myers Squibb outside the submitted work. H.M. McGee reports grants from NIH/NCI K99/R00 CA256526 and Salk Women and Science Special Award during the conduct of the study; personal fees from RefleXion outside the submitted work. R.F. Sweis reports grants and personal fees from BMS, Seattle Genetics, Astellas, Mirati, AstraZeneca, Lilly, Pfizer, Eisai, and Exelixis, grants from Merck, Immunocore, and Moderna, personal fees from Aveo outside the submitted work; in addition, R.F. Sweis has a patent for Neoantigens in Cancer: PCT/US2020/03 pending. B.G. Vincent reports other support from GeneCentric Therapeutics outside the submitted work. R. Zappasodi reports grants from PICI during the conduct of the study; personal fees from Leap Therapeutics outside the submitted work; in addition, R. Zappasodi has a patent for WO2019094352A1 pending, a patent for US20200024350A1 pending and licensed to AGENUS, a patent for US10323091B2 issued and licensed to AGENUS, and a patent for EP3148579B1 issued. V.M. Hubbard Lucey reports personal fees from Bristol Myers Squibb during the conduct of the study; personal fees from Bristol Myers Squibb outside the submitted work. D.K. Wells reports personal fees from Immunai, Rubius Therapeutics, and DeepMind outside the submitted work. T. LaVallee reports personal fees from LisCure Biosciencs, AstraZeneca Stock holder, BiOneCure, Grey Wolf Therapeutics, Immatics, Trex Bio, and Parker Institute of Cancer Immunotherapy outside the submitted work. No disclosures were reported by the other authors.

The authors would like to dedicate this work to oncology patients worldwide whose bravery, patience, and dedication is the true inspiration that fuels oncology research. The authors would like to acknowledge Nina C. Flerin and Massimiliano Mazzone for providing insightful materials, Doreen Valentine for scientific review and discussion, and Cathryn Guevarra for graphic design concepts. Editorial and design support was provided by Stuart Rulten, Liam Jewell, and Matthew Weddig, of Spark Medica Inc, funded by Bristol Myers Squibb. This work originated during a workshop organized by the Parker Institute for Cancer Immunotherapy (PICI), the Cancer Research Institute (CRI), and the Society for Immunotherapy of Cancer (SITC). Continuing support for manuscript preparation was provided by Bristol Myers Squibb. Editorial support was provided by Spark Medica Inc, funded by Bristol Myers Squibb. A.P. Cogdill is supported by the CPRIT Research Training Program (RP170067), the Fulbright Commission Franco-Américaine, and the John J. Kopchick Foundation. C.L. Haymaker is supported by NIH/NCI grants PA30CA016672, P50CA221703, R01CA236905, and U24CA224285. H.M. McGee is supported by NIH/NCI grant K99/R00CA256526. L.H. Butterfield and T. LaVallee are supported by PICI. N.-P. Rudqvist is supported by MDACC intramural funding. R.F. Sweis is supported by NIH K08CA234392. R. Zappasodi is supported by the PICI Bridge Fellows Award and NCI SPORE (P50-CA192937).