This presentation will focus on cancer metabolism and new insights that can be gained from emerging technologies – notably single cell analysis. I will first provide a brief overview of our laboratory's research program around metabolism, emphasizing the research questions we focus on as well as the technologies we've been developing and implementing. I will next discuss work on the development of computational tools using single cell expression data to investigate metabolic networks and pathway activity in single cells to study metabolism in vivo. I will show how this technology can be used to gain insights into metabolism within the tumor microenvironment in human tumors. I will next present an application of this technology in the study a therapeutic our lab has been studying, digoxin – a cardiac glycoside that is under consideration for repurposing in cancer and which we show to target glucose and central carbon metabolism. As a proof of concept, we demonstrate that remodeling of the immune compartment can be achieved upon introduction of this metabolic agent.
References: Sydney M. Sanderson, Zhengtao Xiao, Amy J. Wisdom, Shree Bose, Maria V. Liberti, Michael A. Reid, EmilyHocke, Simon G. Gregory, David G. Kirsch, Jason W. Locasale The Na+/K+ ATPase Regulates Glycolysis and Modifies Immune Metabolism in TumorsbioRxiv 2020.03.31.018739; doi: https://doi.org/10.1101/2020.03.31.018739 Xiao Z, Dai Z, Locasale JW. Metabolic landscape of the tumor microenvironment at single cell resolution. Nature Communications. 2019;10(1):3763. Epub 2019/08/23. doi: 10.1038/s41467-019-11738-0. PubMed PMID: 31434891; PMCID: PMC6704063.
Citation Format: Jason W. Locasale. Cancer metabolism: Emerging insights from single cell analysis [abstract]. In: Abstracts: AACR Virtual Special Conference: Tumor Immunology and Immunotherapy; 2021 Oct 5-6. Philadelphia (PA): AACR; Cancer Immunol Res 2022;10(1 Suppl):Abstract nr IA18.