Expression of Concern: Exploiting Drug Addiction Mechanisms to Select against MAPKi-Resistant Melanoma Free

The editors are publishing this note to alert readers to concerns about this article (1). There are concerns about the assembly of figures presenting clonogenic growth experiments in Figs. 1A, 3E, 3H, 3L, and 6D and Supplementary Figs. S1G, S1K, S2H, S2J, and S6B. In Fig. 1A, M229 DDR4 cells of a 7-day culture with or without inhibitor withdrawal (slow cycling–predominant) were duplicated in M229 DDR4 cells treated with BRAFi+MEKi(+) ERKi(−) and BRAFi+MEKi(−) ERKi(−) as well as in M229 DDR1 cells treated with BRAFi+MEKi(+) ERKi(−) and BRAFi+MEKi(−) ERKi(−) in Supplementary Fig. S1G. In Fig. 1A, M238 DDR1 cells of a 7-day culture with or without an inhibitor (slow cycling–predominant) mirror SKMEL28 DDR1 cells with shVector treated with DMSO in Fig. 3E and vector 500 Off in Supplementary Fig. S1K. SKMEL28 DDR1 cells of a 7-day culture with or without an inhibitor (slow cycling–predominant) in Fig. 1A were also duplicated in vector 500 and BRAF^V600E10 in Supplementary Fig. S1K. In addition, in Fig. 1A, M249 DDR poly cells of a 7-day culture On (cell death–predominant) were duplicated in M249 DDR poly cells treated with BRAFi+MEKi(+) but ERKi(–) in Supplementary Fig. S1G. In Fig. 3H, M249 DDR5 cells treated with DMSO, ATMi (2 µmol/L), and PARPi (0.1 µmol/L) 6 days On match M249 DDR5 cells treated with PARPi+ATMi, Z-DEVD-FMK, ATMi, and +PARPi+Z-DEVD-FMK, and M249 DDR5 cells treated with DMSO, ATMi (2 µmol/L), and PARPi (0.1 µmol/L) 6 days Off match M249 DDR5 cells treated with PARPi+ATMi, Z-DEVD-FMK, and ATMi, +PARPi+Z-DEVD-FMK in Fig. 3L. M229 DDR4 cells treated with PARPi+ATMi, Z-DEVD-FMK, ATMi, and +PARPi+Z-DEVD-FMK Off were duplicated in M229 DDR4 cells treated with staurosporine (+MAPKi) Off, and SKMEL28 DDR1 cells On treated with PARPi+ATMi mirror those treated with PARPi+ATMi, Z-DEVD-FMK, ATMi, and +PARPi+Z-DEVD-FMK in the same line, all in Fig. 3L. In Fig. 6D, BRAFi (1 µmol/L) is rotated in PARPi (2 µmol/L; both in the second Off row). In Supplementary Fig. S2H, M229 DDR4 cells with shVector treated with 5 µmol/L p38i off were duplicated in M229 DDR4 cells with shFRA1 off treated with 0 µmol/L p38i. In addition, in Supplementary Fig. S2H, shFRA1-transduced M229 DDR4 ON cells treated with 0 and 5 µmol/L p38i respectively match M395 DDR poly ON cells transduced with shVector and shAIF #4 in Supplementary Fig. S6B. In Supplementary Fig. S2J, shJunB-transduced SKMEL28 DDR1 Off cells and shJunB-transduced M229 DDR4 Off cells are mirror images.